Quantitative genetics of juvenile hormone esterase, juvenile hormone binding and general esterase activity in the cricket Gryllus assimilis
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منابع مشابه
Purification and characterization of hemolymph juvenile hormone esterase from the cricket, Gryllus assimilis.
Juvenile hormone esterase (JHE) from the serum of the cricket, Gryllus assimilis, was purified to homogeneity in a four-step procedure involving polyethylene glycol precipitation, hydrophobic interaction FPLC, and ion exchange FPLC. This procedure could be completed in 4 days and resulted in a greater than 900-fold purification with greater than 30% recovery. The purified enzyme exhibited a sin...
متن کاملPurification and Characterization of Hemolymph Juvenile Hormone Esterase from the Cricket, <i>Gryllus assimilis</i>
Juvenile hormone esterase (JHE) from the serum of the cricket, Gryllus assimilis, was purifi ed to homogeneity in a four-step procedure involving polyethylene glycol precipitation, hydrophobic interaction FPLC, and ion exchange FPLC. This procedure could be completed in 4 days and resulted in a greater than 900-fold purifi cation with greater than 30% recovery. The purifi ed enzyme exhibited a ...
متن کاملEvolutionary endocrinology of juvenile hormone esterase in Gryllus assimilis: direct and correlated responses to selection.
Hemolymph juvenile hormone esterase (JHE) activity on the third day of the last stadium in the cricket, Gryllus assimilis, exhibited a significant response to selection in each of six replicate lines. Mean realized heritability was 0.26 +/- 0.04. The response was due to changes in whole-organism enzyme activity as well as to changes in the proportion of enzyme allocated to the hemolymph compart...
متن کاملEvolutionary Endocrinology of Juvenile Hormone Esterase: Functional Relationship with Wing Polymorphism in the Cricket, Gryllus Firmus.
The existence, nature, and physiological consequences of genetic variation for juvenile hormone esterase (JHE) activity was studied in the wing-polymorphic cricket, Gryllus firmus. Hemolymph (blood) JHE activity was sixfold lower in nascent short-winged (SW) females, relative to nascent long-winged (LW) females during the last juvenile stadium (stage). Morph-associated genetic variation for JHE...
متن کاملJuvenile hormone esterase: biochemistry and structure.
Normal insect development requires a precisely timed, precipitous drop in hemolymph juvenile hormone (JH) titer. This drop occurs through a coordinated halt in JH biosynthesis and increase in JH metabolism. In many species, JH esterase (JHE) is critical for metabolism of the resonance-stabilized methyl ester of JH. JHE metabolizes JH with a high kcat/KM ratio that results primarily from an exce...
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ژورنال
عنوان ژورنال: Heredity
سال: 1996
ISSN: 0018-067X,1365-2540
DOI: 10.1038/hdy.1996.21